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Cynomolgus Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 113.1 kDa (ITGAV) and 79.0 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gly 22 (ITGB6). The non-reducing (NR) protein migrates as 135-145 kDa (ITGAV) & 85-95 kDa (ITGB6) respectively due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-C52W9) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 0.2-10 ng/mL (QC tested).
Immobilized Fibronectin at 5 μg/mL (100 μL/well) can bind Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-C52W9) with a linear range of 0.078-1.25 μg/mL (Routinely tested).
Fibronectin fragment, premium grade (Cat. No. FIN-H5113) immobilized on CM5 Chip can bind Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT6-C52W9) with an affinity constant of 0.946 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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