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Your Position: Home > Protein > IL-1 alpha > ILA-H82E7

Biotinylated Human IL-1 alpha / IL-1F1 Protein, His,Avitag™ (MALS verified)

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  • Synonym
    IL1A,IL-1 ALPHA,IL1F1,IL1α
  • Source
    Biotinylated Human IL-1 alpha, His,Avitag(ILA-H82E7) is expressed from human 293 cells (HEK293). It contains AA Ser 113 - Ala 271 (Accession # P01583-1).
    Predicted N-terminus: Ser 113
  • Molecular Characterization
    IL-1 alpha Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 21.6 kDa. The protein migrates as 25-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-1 alpha SDS-PAGE

Biotinylated Human IL-1 alpha, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IL-1 alpha MALS images

The purity of Biotinylated Human IL-1 alpha, His,Avitag (Cat. No. ILA-H82E7) is more than 85% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-1 alpha ELISA

Immobilized Biotinylated Human IL-1 alpha, His,Avitag (Cat. No. ILA-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-1 RI Protein, Fc Tag (Cat. No. ILI-H5253) with a linear range of 0.6-10 ng/mL (QC tested).

  • Background
    Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, IL1-ALPHA,IL1F1,and is a cytokine of the interleukin-1 family. IL1a possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum.The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1α possesses biological effect on cells in the picomolar to femtomolar range. In vivo, Shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes.
  • Clinical and Translational Updates

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  • Number of Drugs in Clinical Trials:7 Details
  • Latest Research Phase:Approved

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