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Your Position: Home > Protein > LILRB4 > LI4-HF254

FITC-Labeled Human LILRB4 / CD85k / ILT3 Protein, Fc Tag

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  • Synonym
    LILRB4,ILT3,LIR5,CD85K,HM18
  • Source
    FITC-Labeled Human LILR84, Fc Tag(LI4-HF254) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # AAH26309.1).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    LILRB4 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus

    The protein has a calculated MW of 52.9 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Protein Ratio
    The FITC to protein molar ratio is 1.5-3.5.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LILRB4 SDS-PAGE

FITC-Labeled Human LILR84, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 LILRB4 ELISA

Immobilized Anti-LILRB4 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human LILR84, Fc Tag (Cat. No. LI4-HF254) with a linear range of 0.01-0.313 μg/mL (QC tested).

  • Background
    Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) is also known as CD85 antigen-like family member K (CD85K), Immunoglobulin-like transcript 3 (ILT-3), Leukocyte immunoglobulin-like receptor 5 (LIR-5), Monocyte inhibitory receptor HM18, which belongs to the leukocyte immunoglobulin-like receptor (LIR) family. LILRB4 / CD85K contains 2 Ig-like C2-type (immunoglobulin-like) domains. CD85K is detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. LILRB4 / CD85K is receptor for class I MHC antigens. CD85K recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles, involved in the down-regulation of the immune response and the development of tolerance. LILRB4 interferes with TNFRSF5-signaling and NF-kappa-B up-regulation and inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
  • Clinical and Translational Updates

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ETD of in-stock products: 2 business days

Price(USD) : $319.00

Price(USD) : $2180.00

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  • Number of Drugs in Clinical Trials:5 Details
  • Latest Research Phase:Phase 2 Clinical

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