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Your Position: Home > Protein > Spike RBD > SPD-C82Ef

Biotinylated SARS-CoV-2 Spike RBD Protein (S477N, E484K), His,Avitag™ (MALS verified)

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  • Synonym
    Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD
  • Source
    Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His,Avitag (SPD-C82Ef) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (S477N, E484K)). The mutations (S477N, E484K) were identified in the SARS-CoV-2 variants which emerged in Europe (known as B.1.620).
    Predicted N-terminus: Arg 319
  • Molecular Characterization
    Spike RBD Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 28.2 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike RBD SDS-PAGE

Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Spike RBD MALS images

The purity of Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His,Avitag (Cat. No. SPD-C82Ef) is more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike RBD ELISA

Immobilized Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His,Avitag (Cat. No. SPD-C82Ef) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-2 ng/mL (QC tested).

 Spike RBD ELISA

Immobilized Biotinylated SARS-CoV-2 Spike RBD (S477N, E484K), His,Avitag (Cat. No. SPD-C82Ef) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.1-2 ng/mL (Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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