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SARS-CoV-2 Spike S2-coupled Magnetic Beads

Materials Provided
ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Amount of pre-coupled protein1000 pmol (61.5 μg) Spike S2/mg beads1000 pmol (61.5 μg) Spike S2 /mg beads
Capacity>100 pmol (15 μg) Anti-Spike S2 antibody/mg beads>100 pmol (15 μg) Anti-Spike S2 antibody/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Product FormatPowderPowder
Reconstitution2 mL ultrapure water (1 mg beads / mL)Reconstitute 5 mg / bottle Beads with 5 mL ultrapure water (1 mg beads / mL)
Concentration1 mg beads / mL1 mg beads / mL
  • Background
    The SARS-CoV-2 Spike S2-coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike S2 protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
  • Reconstitution
    See Certificate of Analysis (CoA) for detailed instruction.
  • Storage

    Upon receipt, please store the beads at -20℃ for 1 year in lyophilized state.

    Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

  • Assay Principles
    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

    Antibody Purification:

    1. Resuspend the lyophilized beads by adding the buffer of choice.

    2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.

    3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.

    4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

Typical Data
SARS-CoV-2 Spike S2-coupled Magnetic BeadsSARS-CoV-2 Spike S2-coupled Magnetic Beads (Cat. No. MBS-K018) ELISA bioactivity

Immobilized 61.5 μg S2 protein to 1 mg Beads can bind the Anti-SARS-CoV-2 S2 Antibody with an EC50 of 0.4837 μg/mL (QC tested).

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