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AM130
Human IgG1 | Human Kappa
Biotin
Recombinant Monoclonal
Virus
This product is a specific antibody against SARS-CoV-2 Spike RBD. No cross-reactivity is detected with Spike RBD of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
| Application | Recommended Usage |
| ELISA | 0.1-200 ng/mL |
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Cat. No. S1N-M13L3) is more than 90% and the molecular weight of this protein is around 145-160 kDa verified by SEC-MALS.
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No.S1N-M12A1) at 2 μg/mL, add increasing concentrations of SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H1) and then add Biotinylated Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Cat. No.S1N-M13L3) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.05 ng/mL (QC tested).
Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H2) at 2 μg/mL (100 μL/well) can bind Biotinylated Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Cat. No.S1N-M13L3) with a linear range of 0.195-15.6 ng/mL (Routinely tested).
Immobilized Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (AM359b) (MALS verified)(Cat. No. SPD-M265) at 2 μg/mL, add increasing concentrations of SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (MALS verified)(Cat. No. SPD-C522g) and SARS-CoV-2 Spike Trimer, His Tag (BA.4/Omicron) (MALS verified)(Cat. No. SPN-C5229) and SARS-CoV-2 Spike Trimer, His Tag (BA.5/Omicron) (MALS verified)(Cat. No. SPN-C522e), and then add Biotinylated Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (MALS verified)(Cat. No. S1N-M13L3) at 0.5 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 19.53 ng/mL (Routinely tested).
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