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| ID | Components | Size |
| RAS018-C01 | Pre-coated with SARS-CoV-2 Spike RBD Microplate | 1 plate |
| RAS018-C02 | Anti-SARS-CoV-2 Antibody (Control, Mouse IgG ) | 100 μL |
| RAS018-C03 | HRP-Conjugated Antibody | 10 μg |
| RAS018-C04 | 10 x Washing Buffer | 50 mL |
| RAS018-C05 | Dilution Buffer | 50 mL |
| RAS018-C06 | Substrate Solution | 12 mL |
| RAS018-C07 | Stop Solution | 7 mL |
| RAS018-C08 | SARS-CoV-2 Antibody Positive Control | 100 μL |
| RAS018-C09 | SARS-CoV-2 Antibody Negative Control | 100 μL |
ACROBiosystems, a leading life science company dedicated to aid the global COVID-19 response，developed different kits suitable for serum antibody detection in different species (Mouse, Monkeys, humans) to meet the needs of preclinical and clinical experiments.
This kit is developed for serologic test for Mouse IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Add your sample to the plate, take theAnti-SARS-CoV-2 Antibody (Control, Mouse IgG )as Control sample. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Mouse Anti-SARS-CoV-2 Antibody IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Mouse Anti-SARS-CoV-2 Antibody IgG in mouse serum. Detection was performed using HRP-Conjugated Anti-mouse IgG secondary antibody with sensitivity of 20 ng/mL (QC tested).
Explore our catalog of therapeutic antibody solutions to find the right products for you! We are dedicated to delivering solutions designed to help you drive innovation and push the boundaries of what therapeutic antibodies can be.
We offer a wide range of cell and gene therapy solutions starting from discovery to the clinic. Explore our wide range of proteins, antibodies, kits, and other assays to accelerate the development of your cell and gene therapy.
Organoid Toolbox is a collection of organoid solutions including ready-to-use organoids, organoid differentiation kits, and a variety of services to accelerate the progress of your drug development project.
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GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
A series of immune checkpoints including classic co-inhibitory and co-stimulatory receptors. The comprehensive catalog contains 100+ targets with various species and tags, and the high-quality proteins are in good batch-to-batch consistency.
To meet the needs of ADCs development, ACROBiosystems can provide: A variety of high-quality target proteins; MMPs/Cathepsin/uPA for cleavable linker; Anti-payload antibodies & anti-idiotypic antibodies for immunogenicity and PK analysis; SPR/BLI analytical and ADA development service.
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