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Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag is produced by co-expression of CD79A and CD79B, has a calculated MW of 43.8 kDa (CD79A) and 46.7 kDa (CD79B). Subunit CD79A carries a mouse IgG2a Fc fragment at the C-terminus, followed by a flag tag and subunit CD79B carries a mouse IgG2a Fc fragment at the C-terminus, followed by a polyhistidine tag. The protein migrates as 120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag (Cat. No. CDB-H52W3) is more than 85% and the molecular weight of this protein is around 110-150 kDa verified by SEC-MALS.
Immobilized Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag (Cat. No. CDB-H52W3) at 1 μg/mL (100 μL/well) can bind Polatuzumab with a linear range of 0.1-1 ng/mL (QC tested).
Immobilized Polatuzumab at 1 μg/mL (100 μL/well) can bind Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag (Cat. No. CDB-H52W3) with a linear range of 1-20 ng/mL (Routinely tested).
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