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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 12.0 kDa. The protein migrates as 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human IL-8, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Biotinylated Human IL-8, His,Avitag (Cat. No. IL8-H82H3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human IL-8 Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).
Immobilized Monoclonal Anti-Human IL-8 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-8, His,Avitag (Cat. No. IL8-H82H3) with a linear range of 0.06-0.24 ng/mL (Routinely tested).
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