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Your Position: Home > Protein > NKG2C & CD94 > NC4-H82E3

Biotinylated Human NKG2C&CD94 Protein, His,Avitag™ (MALS verified)

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  • Synonym
    NKG2C & CD94
  • Source
    Biotinylated Human NKG2C&CD94, His,Avitag(NC4-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ile 94 - Leu 231 (NKG2C) & Asp 57 - Ile 179 (CD94) (Accession # P26717-1 (NKG2C) & Q13241-1 (CD94)).
    Predicted N-terminus: Ile 94
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 34.3 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
NKG2C & CD94 SDS-PAGE

Biotinylated Human NKG2C&CD94, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
NKG2C & CD94 MALS images

The purity of Biotinylated Human NKG2C&CD94, His,Avitag (Cat. No. NC4-H82E3) is more than 90% and the molecular weight of this protein is around 50-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
 NKG2C & CD94 ELISA

Immobilized Biotinylated Human NKG2C&CD94, His,Avitag (Cat. No. NC4-H82E3) at 5 μg/mL (100 μL/well) can bind PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein (Cat. No. HLU-HP2H5) with a linear range of 0.01-1.25 μg/mL (QC tested).

Bioactivity-SPR
 NKG2C & CD94 SPR

PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein (Cat. No. HLU-HP2H5) immobilized on CM5 Chip can bind Biotinylated Human NKG2C&CD94, His,Avitag (Cat. No. NC4-H82E3) with an affinity constant of 21.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    CD94 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. NKG2C/CD159c is used as a receptor for NK cells and some cytotoxic T cells to recognize MHC class I HLA-E molecules. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA)-E on target cells.
  • Clinical and Translational Updates

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