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This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 43.7 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE). This protein can bind to VL-Kappa.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Recombinant Protein L, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) is more than 90% and the molecular weight of this protein is around 40-56 kDa verified by SEC-MALS.
Immobilized Mouse Human chimeric Anti-BCMA mAb (Aa01, Mouse IgG1) at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) with a linear range of 0.2-2 ng/mL (QC tested).
Immobilized Ipilimumab at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) with a linear range of 0.2-2 ng/mL (Routinely tested).
Immobilized nivolumab at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) with a linear range of 0.2-2 ng/mL (Routinely tested).
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with an affinity constant of 13.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Anti-CD19 (FMC63), scFv, His Tag with an affinity constant of 74.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Anti-BCMA scFv,His Tag with an affinity constant of 6.56 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) on SA Biosensor, can bind Anti-Human BCMA MAb (human IgG1) with an affinity constant of 14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
2e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Recombinant Protein L, His,Avitag (Cat. No. RPL-P81Q7) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
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