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Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 40.6 kDa (CD3E) and 40.4 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) heterodimer protein migrates as 45-52 kDa due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-C5257) is more than 90% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.
Loaded Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDG-C5257) on AHC Biosensor, can bind Human CD3×BCMA scFV with an affinity constant of 1.02 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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