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This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 27.3 kDa. The protein migrates as 40-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CLEC12A Protein, Avitag,His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
2e5 of anti-CLEC12A CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Human CLEC12A Protein, Avitag,His Tag, premium grade (Cat. No. CLA-H82E6) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Immobilized Biotinylated Human CLEC12A Protein, Avitag,His Tag, premium grade (Cat. No. CLA-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CLEC12A Antibody, Human IgG1 with a linear range of 0.1-0.8 ng/mL (QC tested).
Immobilized Monoclonal Anti-Human CLEC12A Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human CLEC12A Protein, Avitag,His Tag, premium grade (Cat. No. CLA-H82E6) with a linear range of 5-78 ng/mL (Routinely tested).
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