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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 21.0 kDa. The protein migrates as 29-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human TREM2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Biotinylated Human TREM2, His,Avitag (Cat. No. TR2-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (2 μg/well) plate can bind Anti-TREM2 Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (QC tested).
Loaded Biotinylated Human TREM2, His,Avitag (Cat. No. TR2-H82E7) on SA Biosensor, can bind Human APOE3, His Tag (Cat. No. APE-H5246) with an affinity constant of 37.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human TREM2, His,Avitag (Cat. No. TR2-H82E7) on SA Biosensor, can bind Human APOE2 (R154S), His Tag (Cat. No. APE-H5256) with an affinity constant of 2.08 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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