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Items | Size (5mg) | Size(5mg X 2) |
Particle size | 2 μm | 2 μm |
Physical appearance | Powder mixture | Powder mixture |
Amount of Coupled Protein | ≈1000 pmol/mg Beads | ≈1000 pmol/mg Beads |
Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
Reconstitution | 5 mL sterile deionized water (1 mg beads/mL) | 5 mL sterile deionized water (1 mg beads/mL) |
See Certificate of Analysis (CoA) for detailed instruction.
Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated protein can bind to the SA beads irreversibly. The Magenetic beads are pre-coupled with Streptavidin tetramer protein and blocked with BSA, it is a ready-to-use beads, which can be used for binding biotinylated proteins and antibodies.
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Immobilized 60 μg Human uPAR Protien to 1mg Beads add sample Mouse Anti-uPAR antibody Antibody, detection PE anti-Mouse IgG1 Fc with an EC50 0.35 μg/mL (QC tested).
Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads add sample Anti-SARS-CoV-S protein RBD Antibody, detection Goat anti-Human IgG Fc Secondary Antibody, PE, with an EC50 0.1750 μg/mL (Routinely tested).
Freeze-thaw stability test. Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads, after different freeze-thaw cycles, add sample Anti-SARS-CoV-S protein RBD Antibody, detection Goat anti-Human IgG Fc Secondary Antibody, PE, showed little deviation from the unfreeze-thaw sample (RSD<10%). Three freeze-thaw cycles were performed (Routinely tested).
Accelerated stability test. Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads, after placing the lyophilized beads at 37°C for 7 daysadd sample Anti-SARS-CoV-S protein RBD Antibody, detection Goat anti-Human IgG Fc Secondary Antibody, PE, showed little deviation from the unfreeze-thaw sample (RSD<10%). Data were measured on day 0, 3, 7 respectively (Routinely tested).
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