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Items | Specifications |
Material | Polystyrene |
Color | Clear |
Plate Blocking | 2% BSA Blocking Buffer |
Formulations | Clear, 96-well plates, coated with 100uL of streptavidin tetramer and blocked with 200uL of 2% BSA Blocking Buffer |
Detection Method | Colorimetric |
Capacity | ~5pmol biotin/well |
CV% of plates/wells | < 10% |
Type | Detection Plate, Immunoassay, ELISA |
Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. The Streptavidin Coated Plates we provide are easy to use and widely available for applications.
Immobilized Biotinylated 2019-nCoV S protein RBD at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Anti-SARS-CoV-S protein RBD with a linear range of 0.025-1.5625 ng/mL (QC tested).
Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (Routinely tested).
Immobilized Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat. No. SP-11), can bind Human PD-L1, mFc Tag with a linear range of 0.4-6.25 ng/mL (Routinely tested).
Routine stability test. After placing the Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat.No. SP-11) at 2-8°C for 24 months, binding activity between the SP-11,the Spike RBD Protein and the Anti-SARS-CoV-2 Spike RBD Antibody showed no difference from the Plate Coated Before the test.
Cat. No. SP-11, Adsorption stability test of avidin protein. After three SA plates were washed with buffer solution for three times, five times and ten times respectively, binding activity between the SA plate, the Spike RBD Protein and the Anti-SARS-CoV-2 Spike RBD Antibody showed no difference. The test shows that the SA protein has good stability on the plate.
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