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AS35
Human IgG1 | Kappa
Unconjugated
Recombinant Monoclonal
Virus
This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
| Application | Recommended Usage |
| ELISA | 0.2-50 ng/mL |
>95% as determined by SDS-PAGE.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Serial dilutions of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35) (Cat.No. SAD-S35) was detected by SARS-CoV-2 Inhibitor screening Kit with a half maximal inhibitory concentration (IC50) of 1.472 μg/mL (QC tested).
Immobilized SARS-CoV-2 S1 protein, His Tag at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35) (Cat. No. SAD-S35) with a linear range of 0.2-1.95 ng/mL (QC tested).
Serial dilutions of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35) (Cat.No. SAD-S35) was detected by Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit with a half maximal inhibitory concentration (IC50) of 0.6265 μg/mL (Routinely tested).
Serial dilutions of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35) (Cat. No. SAD-S35) was incubated with SARS-CoV-2 pseudotyped virus at 37 °C for 1 hour. Afterward, Huh-7 cells were added, followed by incubation at 37 °C for 24h. Chemiluminescence detection was performed and the virus neutralization titers (IC50) is 2.181 nM using the Reed-Muench method.
Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35) (Cat.No. SAD-S35) neutralizes SARS-CoV-2 Spike RBD by inhibiting RBD:ACE2 interaction. The ACE2-coated plate is incubated with the wild type (WT) RBD or B.1.1.7, B.1.351, P.1, B.1.617.1 mutant and treated with the antibody at increasing concentration. Percent inhibition is calculated based on the OD value.
Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35) (Cat. No. SAD-S35) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD (A475V), His Tag with an affinity constant of 87.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
FACS analysis shows that the binding of SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat. No. SPD-C5259) to Vero E6 cells surface ACE2 was inhibited by increasing concentration of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35) (Cat. No. SAD-S35). The concentration of SARS-CoV-2 S protein RBD used is 5μg/ml. The IC50 is 10.33 μg/ml (Routinely tested).
FACS analysis shows that the binding of SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) to Vero E6 cells surface ACE2 was inhibited by increasing concentration of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35) (Cat. No. SAD-S35). The concentration of SARS-CoV-2 S1 protein used is 3ug/ml. The IC50 is 1.352 μg/ml (Routinely tested).
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