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Your Position: Home > Protein > Complement C5 > CO5-H52H7

Human Complement C5 (w917s) Protein, His Tag (MALS verified)

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  • Synonym
    Complement C5,C5,CPAMD4
  • Source
    Human Complement C5 (w917s), His Tag(CO5-H52H7) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # P01031-1 (W917S)).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    Complement C5 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 188.1 kDa. The protein migrates as 70 kDa and 120-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Complement C5 SDS-PAGE

Human Complement C5 (w917s), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Complement C5 MALS images

The purity of Human Complement C5 (w917s), His Tag (Cat. No. CO5-H52H7) is more than 85% and the molecular weight of this protein is around 180-225 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Complement C5 ELISA

Immobilized Human Complement C5 (w917s), His Tag (Cat. No. CO5-H52H7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).

 Complement C5 ELISA

Immobilized Human Complement C5 (w917s), His Tag (Cat. No. CO5-H52H7) at 2 μg/mL (100 μL/well) can bind Eculizumab Biosimilar with a linear range of 0.2-16 ng/mL (Routinely tested).

  • Background
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
  • Clinical and Translational Updates

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