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This protein carries a polyhistidine tag at the C-terminus.
The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human HGF Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human HGF Protein, His Tag (Cat. No. HGF-H52H3) is more than 90% and the molecular weight of this protein is around 90-105 kDa verified by SEC-MALS.
Human EGF (Cat. No. EGF-H52H3), Noggin (Cat. No. NON-H5257), R-spondin1 (Cat. No. RS6-H4220), FGF7 (Cat. No. FG7-H52H5), FGF10, HGF (Cat. No. HGF-H52H3) actively support liver ductal organoid growth.
Immobilized Human HGF Protein, His Tag (Cat. No.HGF-H52H3 ) at 2 μg/mL (100 μL/well) can bind Human HGF R, Fc Tag (Cat. No. MET-H5256) with a linear range of 1-39 ng/mL (QC tested).
Human HGF R, Fc Tag (Cat. No. MET-H5256) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF Protein, His Tag (Cat. No. HGF-H52H3) with an affinity constant of 0.486 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Human HGF R, Fc Tag (Cat. No. MET-H5256) on Protein A Biosensor, can bind Human HGF Protein, His Tag (Cat. No. HGF-H52H3) with an affinity constant of 3.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Human HGF Protein, His Tag (Cat. No. HGF-H52H3) stimulates the secrection IL-11 by Saos-2 cells. The specific activity of Human HGF Protein, His Tag is >6.00 x 10^5 IU/mg, which is calibrated against WHO Hepatocyte Growth Factor (precursor) (Human rDNA derived) (NIBSC code: 96/556) (QC tested).
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Price(USD) : $380.00
Price(USD) : $1800.00
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