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Your Position: Home > Protein > Siglec-8 > SI8-H52H8

Human Siglec-8 Protein, His Tag (MALS verified)

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  • Synonym
    Siglec-8,SAF-2,SIGLEC8,SAF2
  • Source
    Human Siglec-8, His Tag (SI8-H52H8) is expressed from human 293 cells (HEK293). It contains AA Met 17 - Ala 363 (Accession # Q9NYZ4-1).
    Predicted N-terminus: Met 17
  • Molecular Characterization
    Online(Met 17 - Ala 363) Q9NYZ4-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 39.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS with Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Siglec-8, His Tag (Cat. No. SI8-H52H8) SDS-PAGE gel

Human Siglec-8, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Siglec-8, His Tag (Cat. No. ) MALS images

The purity of Human Siglec-8, His Tag (Cat. No. SI8-H52H8) is more than 85% and the molecular weight of this protein is around 102-125 kDa verified by SEC-MALS.

  • Background
    Siglec-8 is also known as SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L and sialic acid binding Ig like lectin 8. Siglec-8 was first identified by CD33 homology screening of ESTs from a cDNA library generated from a patient diagnosed with idiopathic hypereosinophilic syndrome and was originally termed SAF-2 (sialoadhesin family 2). At the tissue level, Siglec-8 mRNA was found to be most highly expressed in lung, PBMCs, spleen, and kidney. Two splice variants of Siglec-8 exist. The initially characterized form contains 431 amino acid residues in total, subsequently, a longer form of Siglec-8, initially termed Siglec-8L. Both forms of Siglec-8 are found in eosinophils and contain a V-set domain with lectin activity and two C2-type Ig repeat domains in the extracellular region.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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