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Your Position: Home > Protein > DLL3 > DL3-H52H4

Human DLL3 Protein, His Tag (MALS verified)

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  • Synonym
    Delta3,delta-like 3 (Drosophila),delta-like protein 3,DLL3,Pudgy,SCDO1,SCDO1delta3
  • Source
    Human DLL3, His Tag(DL3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Leu 492 (Accession # Q9NYJ7-1).
    Predicted N-terminus: Ala 27
  • Molecular Characterization
    DLL3 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 50.4 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS with Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
DLL3 SDS-PAGE

Human DLL3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
DLL3 MALS images

The purity of Human DLL3, His Tag (Cat. No. DL3-H52H4) is more than 95% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

Bioactivity-ELISA
 DLL3 ELISA

Immobilized Human DLL3, His Tag (Cat. No. DL3-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-DLL3 Antibody (specific Binding DSL of DLL3), Human IgG1 with a linear range of 0.2-2 ng/mL (QC tested).

 DLL3 BATCH ELISA
 DLL3 ELISA

Immobilized Human DLL3, His Tag (Cat. No. DL3-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-DLL3 Antibody (specific Binding EGFs of DLL3), Human IgG1 with a linear range of 0.01-2 ng/mL (Routinely tested).

Bioactivity-SPR
 DLL3 SPR

Bispecific T-cell Engager captured on Protein A Chip can bind Human DLL3, His Tag (Cat. No. DL3-H52H4) with an affinity constant of 1.14 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands.May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. DLL3 protein is expressed on the surface of tumor cells but not in normal adult tissues.
  • Clinical and Translational Updates

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