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Your Position: Home > Protein > CD47 > CD7-M522b

Mouse CD47 Protein, His Tag (MALS verified)

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  • Synonym
    CD47,MER6,IAP,OA3
  • Source
    Mouse CD47, His Tag(CD7-M522b) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Lys 140 (Accession # Q61735-1).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    CD47 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 15.7 kDa. The protein migrates as 28-42 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.5M Arginine, PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD47 SDS-PAGE

Mouse CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD47 MALS images

The purity of Mouse CD47, His Tag (Cat. No. CD7-M522b) is more than 90% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD47 ELISA

Immobilized Mouse SIRP alpha Protein, Mouse IgG2a Fc Tag (Cat. No. SIA-M5252) at 10 μg/mL (100 μL/well) can bind Mouse CD47, His Tag (Cat. No. CD7-M522b) with a linear range of 0.078-5 μg/mL (QC tested).

Bioactivity-SPR
 CD47 SPR

Mouse SIRP alpha, His Tag (Cat. No. SIA-M52H4) immobilized on CM5 Chip can bind Mouse CD47, His Tag (Cat. No. CD7-M522b) with an affinity constant of 2.52 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
  • Clinical and Translational Updates

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