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Your Position: Home > Protein > Frizzled-2 > FZ2-H5255

Human Frizzled-2 / FZD2 (24-190) Protein, Fc Tag

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  • Synonym
    FZD2,Frizzled-2,Fz-2,hFz2,FzE2
  • Source
    Human Frizzled-2, Fc Tag(FZ2-H5255) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Pro 190 (Accession # Q14332-1).
    Predicted N-terminus: Gln 24
  • Molecular Characterization
    Frizzled-2 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 44.4 kDa. The protein migrates as 50-62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Frizzled-2 SDS-PAGE

Human Frizzled-2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 Frizzled-2 ELISA

Immobilized Human Frizzled-2, Fc Tag (Cat. No. FZ2-H5255) at 1 μg/mL (100 μL/well) can bind Wnt surrogate Human IgG1(D239E,L241M) | Human Lambda with a linear range of 0.1-8 ng/mL (QC tested).

  • Background
    Frizzled-2 (FZD2) is also known as FzE2, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD2 contains one FZ (frizzled) domain. FZD2 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. The Lys-Thr-X-X-X-Trp motif of FZD2 interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
  • Clinical and Translational Updates

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  • Number of Drugs in Clinical Trials:1 Details
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