Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Protein > Fc gamma RIIA / CD32a > CDA-H82E6

Biotinylated Human Fc gamma RIIA / CD32a (H167) Protein, Avitag™,His Tag (SPR & BLI & MALS verified)

Order Now

  • Synonym
    CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2
  • Source
    Biotinylated Human CD32a (H167), Avitag,His Tag(CDA-H82E6) is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1).
    Predicted N-terminus: Ala 36
  • Molecular Characterization
    Fc gamma RIIA / CD32a Structure

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 24.0 kDa. The protein migrates as 30-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Fc gamma RIIA / CD32a SDS-PAGE

Biotinylated Human CD32a (H167), Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Fc gamma RIIA / CD32a MALS images

The purity of Biotinylated Human CD32a (H167), Avitag,His Tag (Cat. No. CDA-H82E6) is more than 90% and the molecular weight of this protein is around 30-55 kDa verified by SEC-MALS.

Bioactivity-SPR
 Fc gamma RIIA / CD32a SPR

Biotinylated Human CD32a (H167), Avitag,His Tag (Cat. No. CDA-H82E6) captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 0.66 μM as determined in a SPR assay (Biacore T200) (QC tested).

 Fc gamma RIIA / CD32a SPR

Immobilized Biotinylated Human CD32a (H167), Avitag,His Tag (Cat. No. CDA-H82E6) on SA Chip can bind Rituximab with an affinity constant of 0.74 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
 Fc gamma RIIA / CD32a BLI

Loaded Biotinylated Human CD32a (H167), Avitag,His Tag (Cat. No. CDA-H82E6) on SA Biosensor, can bind Rituximab with an affinity constant of 0.59 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized.
    There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
  • Clinical and Translational Updates

Comments (0)


ETA of in-stock products: 2 business days

Price(USD) : $250.00

Price(USD) : $1080.00

Recommended Products
Streptavidin Coated Plates

Price(USD) : $32.00

Price(USD) : $137.00

Streptavidin-HRP

Price(USD) : $50.00

Price(USD) : $120.00

Magnetic Beads™ Streptavidin

Price(USD) : $138.00

Price(USD) : $230.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Phase 1 Clinical

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message