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Your Position: Home > Protein > Fc gamma RIIA / CD32a > CD1-H5223

Human Fc gamma RIIA / CD32a (H167) Protein, His Tag (SPR & BLI & MALS verified)

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  • Synonym
    CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2
  • Source
    Human CD32a (H167), His Tag (CD1-H5223) is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1).
    Predicted N-terminus: Ala 36
  • Molecular Characterization
    Online(Ala 36 - Ile 218) P12318-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 21.5 kDa. The protein migrates as 29-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD32a (H167), His Tag (Cat. No. CD1-H5223) SDS-PAGE gel

Human CD32a (H167), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human CD32a (H167), His Tag (Cat. No. ) MALS images

The purity of Human CD32a (H167), His Tag (Cat. No. CD1-H5223) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Human CD32a (H167), His Tag (SPR & BLI verified) (Cat. No. CD1-H5223) on CM5 Chip via anti-His antibody, can bind  Rituximab with an affinity constant of 1.45 μM as determined in a SPR assay (Biacore T200) (QC tested).

Human_FcRn_Heterodimer_Protein_SPR

Rituximab immobilized on CM5 Chip can bind Human CD32a (H167), His Tag (Cat. No. CD1-H5223) with an affinity constant of 0.882 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CD32a (H167), His Tag (SPR & BLI verified) (Cat. No. CD1-H5223) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 1.30 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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