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Your Position: Home > Protein > Fetuin B > FEB-H52H7

Human Fetuin B Protein, His Tag

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  • Synonym
    FETUB,Fetuin-B,16G2,Gugu
  • Source
    Human Fetuin B, His Tag(FEB-H52H7) is expressed from human 293 cells (HEK293). It contains AA Cys 16 - Pro 382 (Accession # AAH69670 ).
    Predicted N-terminus: Cys 16
  • Molecular Characterization
    Fetuin B Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 42.4 kDa. The protein migrates as 53-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Fetuin B SDS-PAGE

Human Fetuin B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Fetuin-B (FETUB), a second member of the fetuin family in mammals, is also known as Fetuin-like protein IRL685, 16G2 and Gugu, which is expressed in liver and testis and contains 2 cystatin fetuin-B-type domains. FETUB is a protease inhibitor required for egg fertilization. FETUB is also required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity). As a liver-derived plasma protein, FETUB is essential for fertilization.
  • Clinical and Translational Updates

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