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Your Position: Home > Protein > GITR > GIR-H5228

Human GITR / TNFRSF18 Protein, His Tag (MALS verified)

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  • Synonym
    AITR,GITR,TNFRSF18,CD357
  • Source
    Human GITR, His Tag(GIR-H5228) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Glu 161 (Accession # Q9Y5U5-1).
    Predicted N-terminus: Gln 26
  • Molecular Characterization
    GITR Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.4 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
GITR SDS-PAGE

Human GITR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 92%.

SEC-MALS
GITR MALS images

The purity of Human GITR, His Tag (Cat. No. GIR-H5228) is more than 90% and the molecular weight of this protein is around 18-25 kDa verified by SEC-MALS.

Bioactivity-ELISA
 GITR ELISA

Immobilized Biotinylated Human GITR Ligand, Avitag,Fc Tag (Cat. No. GIL-H82F8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human GITR, His Tag (Cat. No. GIR-H5228) with a linear range of 0.6-39 ng/mL (QC tested).

  • Background
    Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.
  • Clinical and Translational Updates

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Price(USD) : $319.00

Price(USD) : $2400.00

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