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Your Position: Home > COVID-19 R&D > One jab and you’re done! CanSino and Johnson & Johnson adenovirus vaccines receive regulatory approval.
One jab and you’re done! CanSino and Johnson & Johnson adenovirus vaccines receive regulatory approval.
Release time: 2021-03-08 Source: ACROBiosystems Read: 1559

On February 25, the Chinese NMPA official website announced that the State Food and Drug Administration conditionally approved the CanSino biological AG adenoviral vaccine to market. This is the third COVID-19 vaccine approved for marketing in China and the first approved adenovirus vector-based vaccine. Prior to this, CanSino had obtained emergency use authorizations (EUAs) from Pakistan and Mexico. Within days, the FDA unanimously granted EUA to Johnson & Johnson’s vaccine for people over the age of 18. This is the third new COVID-19 vaccine approved in the United States and the first approved adenovirus type 26 vector-based vaccine.

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Figure 1. Johnson & Johnson application presented to the FDA expert panel


 Request for the slides


These two adenovirus vector-based vaccines require only one shot to provide protection which will vastly simplify vaccination campaign compared to two shot regiment needed for inactivated and mRNA based vaccines. Additionally, the adenovirus vaccine does not require cryogenic temperature for storage enabling vaccination campaigns in developing countries.

According to the published Phase 3 clinical trials provided by CanSino, the vaccine protected 65.28% patients against mild symptoms 28 days after single injection, while the protective effect against severe illness 14 days after injection was determined to be 90.07%. The overall protective efficacy of the vaccine is 68.83% and 95.47% for mild and severe cases respectively.

Johnson and Johnson’s FDA application also disclosed their phase III data. The phase III clinical trial included 44,325 participants worldwide of which 47% were from the United States, 40% were from Latin America (17% Brazilian variant), and 13% from South Africa. Of all the participants 24% are seniors over 65 and 40% have at least one chronic underlying disease. After 14 days from injection, the vaccine provided protection to 66.9% of patients against moderate to critical cases of COVID-19. 28 days after receiving the single injection, the protection effect stayed unchanged at 66.1%. This vaccine was also shown to be effective for the elderly and little difference in efficacy among people with underlying diseases and young participants without underlying diseases was observed. Furthermore, Johnson & Johnson also presented data on protective effect of this vaccine against South Africa and Brazilian variants. In the United States, where the original D614G strain is dominant, the vaccine efficacy is 72% (severe symptom protection 86%). In South Africa, where the B.1.351 variant strain account for 95% of all infections, the vaccine efficacy dropped to 64% (severe symptom protection 82%). Against Brazilian P.2 variant, where the strains account for nearly 70% of all infections, the vaccine efficacy is 68% (severe symptom protection 88% ) (Figure 2 ). Overall, the Johnson & Johnson's vaccine displays strong protection against both South African and Brazilian variant comparable to the original strain. This finding is a good news particularly due to concerns of resurgence of cases due to immune escape of the variants.

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Figure 2. Summary of the protective effect of Johnson & Johnson’s vaccine on the new coronavirus mutant strain


The approval of CanSino and Johnson & Johnson's vaccines adds to the arsenal against COVID-19 particularly with the positive data against variants.

As part of the vaccine effectiveness data, Johnson and Johnson presented neutralizing antibody titers produced in the human body 14 days and 28 days after vaccination. Neutralizing antibody is a type of specific antibody produced in immune response that blocks pathogens from invading host cells. Therefore, the presence of neutralizing antibodies in vaccinated sera translates to the clinical protective efficacy of the vaccine.
 At present, the gold standard neutralizing antibody detection is Plaque Reduction Neutralization Titer (PRNT) assay based on live viruses and cells performed in the P3 laboratory setting. Due to the complexity, requirement and slow throughput of the assay, surrogate neutralization assay is commonly used to detect neutralizing antibodies. These neutralization assay can be readily used in assessing neutralizing antibody titer of vaccinated population. At present, the main methods used to detect neutralizing antibodies are chemiluminescence, colloidal gold and enzyme-linked immunoassays (ELISA) each with its own unique advantages. ELISAs can be used for qualitative and semi-quantitative detection of neutralizing antibodies with high sensitivity and high throughput. Currently, the only FDA approved SARS-CoV-2 neutralizing antibody detection kit is an ELISA. Experimental data shows that it is highly correlated to neutralizing antibody titers using real and pseudovirus based PRNT assay.

 

> > > > Click to see more evaluation of core vaccine reagent


ACROBiosystems provides high-quality neutralizing antibody detection kit raw materials, including ACE2 protein, RBD protein and neutralizing antibody standards. ACROBiosystems also has a neutralization antibody ELISA kit (TAS-K022) for research use only applications.

Category

Cat. No.

Product description

ACE2 protein

AC2-H5255

Human ACE2 / ACEH Protein, Fc Tag (MALS verified)

AC2-H82E7

Biotinylated Human ACE2 / ACEH Protein, His,Avitag™

RBD protein

SPD-C5251

SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag

SPD-C5255

SARS-CoV-2 (COVID-19) S protein RBD, Fc Tag (MALS verified)

SPD-C52M5

SARS-CoV-2 S protein RBD trimer, His Tag (MALS verified)

Neutralizing antibody

SPD-M180

Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Human IgG1 (AM180)

SPD-M128

Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128)

S1N-M12A1

Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (Trehalose free)


> > > > Click for more diagnostic material


The addition of two new vaccines from CanSino and Johnson and Johnson provides optimism in lowering SARS-CoV-2 infection and COVID-19 cases by the end of the year. The current burden of SARS-CoV-2 is most acutely faced by North and South America and Europe followed by South East Asia according to the CDC.
 

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Figure 3.  CDC data on global trend of COVID-19 pandemic


However, the emerging SARS-CoV-2 variant presents a high risk of pandemic resurgence and needs to be assessed during vaccine and therapeutic development.  


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Figure 4  Key mutation sites of interest


ACROBiosystem is closely tracking these emerging variants and offers S trimer, RBD and NTD mutants based on the three leading variants with variety of tags. Single S protein mutants are also available for ordering.  

 
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Product list
  • Spike mutants from critical variants

  • All Spike mutants

MoleculeCat.No.TagHostProduct descriptionVariantPreorder/Order
S proteinSPN-C52HcHis TagHEK293SARS-CoV-2 S protein (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G, A701V), His TagB.1.351

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S proteinSPN-C52HkHis TagHEK293SARS-CoV-2 (COVID-19) S protein (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V), His TagB.1.351

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S proteinSPN-C82E4His & Avi TagHEK293Biotinylated SARS-CoV-2 S protein (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V), His,Avitag™B.1.351

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S proteinSPN-C52H6His TagHEK293SARS-CoV-2 (COVID-19) S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His TagB.1.1.7

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S proteinSPN-C82E5His & Avi TagHEK293Biotinylated SARS-CoV-2 S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His,Avitag™B.1.1.7

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S proteinSPN-C52HgHis TagHEK293SARS-CoV-2 (COVID-19) S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F), His TagP.1

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S proteinSPN-C82E6His & Avi TagHEK293Biotinylated SARS-CoV-2 S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F), His,Avitag™P.1

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S proteinSPN-C52H3His TagHEK293SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified)B.1.1.7/B.1.351/P.1

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S proteinSPN-C82E3His & Avi TagHEK293Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag™, Super stable trimer (MALS verified)B.1.1.7/B.1.351/P.1

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S1 proteinS1N-C52HdHis TagHEK293SARS-CoV-2 (COVID-19) S1 protein (HV69-70del), His TagB.1.1.7

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S1 proteinS1N-C52HcHis TagHEK293SARS-CoV-2 (COVID-19) S1 protein (Y144del), His TagB.1.1.7

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S1 proteinS1N-C52HbHis TagHEK293SARS-CoV-2 (COVID-19) S1 protein (P681H), His TagB.1.1.7

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S1 proteinS1N-C52HkHis TagHEK293SARS-CoV-2 (COVID-19) S1 protein (HV69-70del, N501Y, D614G), His TagB.1.1.7

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S1 proteinS1N-C52HrHis TagHEK293SARS-CoV-2 (COVID-19) S1 protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H), His TagB.1.1.7

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S1 proteinS1N-C52HmHis TagHEK293SARS-CoV-2 S1 protein (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G), His TagB.1.351

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S1 proteinS1N-C52HgHis TagHEK293SARS-CoV-2 (COVID-19) S1 protein (N501Y), His TagB.1.1.7/B.1.351/P.1

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S1 proteinS1N-C5256His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (D614G), His TagB.1.1.7/B.1.351/P.1

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S1 proteinS1N-C52HpHis TagHEK293SARS-CoV-2 (COVID-19) S1 protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y), His TagP.1

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S1 proteinS1N-C52HnHis TagHEK293SARS-CoV-2 S1 protein (E484K, D614G), His TagB.1.351/P.1

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S1 proteinS1N-C82E3His & Avi TagHEK293Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His,Avitag™B.1.1.7/B.1.351/P.1

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S1 proteinS1N-C52H9His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (A570D), His TagB.1.1.7

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S1 protein NTDS1D-C52HdHis TagHEK293SARS-CoV-2 (COVID-19) S1 protein NTD (HV69-70del, Y144del), His TagB.1.1.7

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S1 protein NTDS1D-C52H9His TagHEK293SARS-CoV-2 (COVID-19) S1 protein NTD (L18F, D80A, D215G, R246I), His TagB.1.351

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S1 protein NTDS1D-C52HcHis TagHEK293SARS-CoV-2 (COVID-19) S protein NTD (L18F, D80A, D215G, 242-244del, R246I), His TagB.1.351

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S1 protein NTDS1D-C52HeHis TagHEK293SARS-CoV-2 (COVID-19) S1 protein NTD (L18F, T20N, P26S, D138Y, R190S), His TagP.1

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S protein RBDSPD-C82E6His & Avi TagHEK293Biotinylated SARS-CoV-2 S protein RBD (N501Y), His,Avitag™B.1.1.7/B.1.351/P.1

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S protein RBDSPD-C52HsHis TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (K417N), His TagB.1.351

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S protein RBDSRD-C52H3His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (E484K), His TagB.1.351/P.1

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S protein RBDSPD-C52HpHis TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y), His TagB.1.351

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S protein RBDSPD-C82E5His & Avi TagHEK293Biotinylated SARS-CoV-2 S protein RBD (K417N,E484K,N501Y), His,Avitag™B.1.351

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S protein RBDSPD-C52HnHis TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (N501Y), His TagB.1.1.7/B.1.351/P.1

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S protein RBDSPD-C52HtHis TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (K417T), His TagP.1

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S protein RBDSPD-C52HrHis TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (K417T, E484K, N501Y), His TagP.1

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S protein RBDSPD-C82E7His & Avi TagHEK293Biotinylated SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), His,Avitag™P.1

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S2 proteinS2N-C52HdHis TagHEK293SARS-CoV-2 (COVID-19) S2 protein (T716I, S982A, D1118H), His TagB.1.1.7

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S2 proteinS2N-C52HcHis TagHEK293SARS-CoV-2 (COVID-19) S2 protein (A701V), His TagB.1.351

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S2 proteinS2N-C52HeHis TagHEK293SARS-CoV-2 (COVID-19) S2 protein (T1027I, V1176F), His TagP.1

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Nucleocapsid proteinNUN-C52H8His TagHEK293SARS-CoV-2 Nucleocapsid protein (D3L, S235F), His TagB.1.1.7

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MoleculeCat.No.SpeciesTagHostProduct descriptionPreorder/Order
S proteinSPN-C52H6SARS-CoV-2  (B.1.1.7)His TagHEK293SARS-CoV-2 (COVID-19) S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His Tag

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S proteinSPN-C82E5SARS-CoV-2  (B.1.1.7)His Tag & Avi TagHEK293Biotinylated SARS-CoV-2 S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His,Avitag™

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S proteinSPN-C52H3SARS-CoV-2  (B.1.1.7/B.1.351/P.1)His TagHEK293SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified)

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S proteinSPN-C82E3SARS-CoV-2  (B.1.1.7/B.1.351/P.1)His Tag & Avi TagHEK293Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag™, Super stable trimer (MALS verified)

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S proteinSPN-C52HcSARS-CoV-2  (B.1.351)His TagHEK293SARS-CoV-2 S protein (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G, A701V), His Tag

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S proteinSPN-C52HkSARS-CoV-2  (B.1.351)His TagHEK293SARS-CoV-2 (COVID-19) S protein (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V), His Tag

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S proteinSPN-C82E4SARS-CoV-2  (B.1.351)His Tag & Avi TagHEK293Biotinylated SARS-CoV-2 S protein (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V), His,Avitag™

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S proteinSPN-C52HgSARS-CoV-2  (P.1)His TagHEK293SARS-CoV-2 (COVID-19) S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F), His Tag

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S proteinSPN-C82E6SARS-CoV-2  (P.1)His Tag & Avi TagHEK293Biotinylated SARS-CoV-2 S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F), His,Avitag™

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S proteinSPN-C52HdSARS-CoV-2His TagHEK293SARS-CoV-2 S protein (F817P, A892P, A899P, A942P, K986P, V987P), His Tag

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S1 proteinS1N-C52HdSARS-CoV-2  (B.1.1.7)His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (HV69-70del), His Tag

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S1 proteinS1N-C52HcSARS-CoV-2  (B.1.1.7)His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (Y144del), His Tag

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S1 proteinS1N-C52HfSARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (E583D), His Tag

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S1 proteinS1N-C52HnSARS-CoV-2  (B.1.351/P.1)His TagHEK293SARS-CoV-2 S1 protein (E484K, D614G), His Tag

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S1 proteinS1N-C52HrSARS-CoV-2  (B.1.1.7)His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H), His Tag

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S1 proteinS1N-C52HpSARS-CoV-2  (P.1)His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y), His Tag

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S1 protein NTDS1D-C52H8SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S1 protein NTD (A222V), His Tag

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S1 protein NTDS1D-C52H7SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S1 protein NTD (N234Q), His Tag

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S1 protein NTDS1D-C52H5SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S1 protein NTD (A262S), His Tag

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S1 protein NTDS1D-C52H4SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S1 protein NTD (P272L), His Tag

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S1 protein NTDS1D-C52H9SARS-CoV-2  (B.1.351)His TagHEK293SARS-CoV-2 S1 protein NTD (L18F, D80A, D215G, R246I), His Tag

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S1 protein NTDS1D-C52HdSARS-CoV-2  (B.1.1.7)His TagHEK293SARS-CoV-2 (COVID-19) S1 protein NTD (HV69-70del, Y144del), His Tag

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S1 protein NTDS1D-C52HcSARS-CoV-2  (B.1.351)His TagHEK293SARS-CoV-2 (COVID-19) S protein NTD (L18F, D80A, D215G, 242-244del, R246I), His Tag

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S1 protein NTDS1D-C52HeSARS-CoV-2  (P.1)His TagHEK293SARS-CoV-2 (COVID-19) S1 protein NTD (L18F, T20N, P26S, D138Y, R190S), His Tag

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S protein RBDSPD-S52H5SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (N354D), His Tag

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S protein RBDSPD-S52H4SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag

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S protein RBDSPD-S52H8SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (R408I), His Tag

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S protein RBDSPD-C52HsSARS-CoV-2  (B.1.351)His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (K417N), His Tag

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S protein RBDSPD-C52HtSARS-CoV-2  (P.1)His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (K417T), His Tag

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S protein RBDSPD-S52H7SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag

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S protein RBDSPD-C52HgSARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (N439K), His Tag

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S protein RBDSRD-C52H2SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (N440K), His Tag

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S protein RBDSPD-C52HeSARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (L452R), His Tag

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S protein RBDSPD-C52HkSARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (Y453F), His Tag

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S protein RBDSPD-C52HdSARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (A475V), His Tag

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S protein RBDSPD-C52H4SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (G476S), His Tag

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S protein RBDSPD-C52HmSARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (S477N), His Tag

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S protein RBDSPD-C52H5SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (V483A), His Tag

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S protein RBDSRD-C52H3SARS-CoV-2  (B.1.351/P.1)His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (E484K), His Tag

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S protein RBDSPD-C52HfSARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (F490L), His Tag

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S protein RBDSPD-C82E6SARS-CoV-2  (B.1.1.7/B.1.351/P.1)His Tag & Avi TagHEK293Biotinylated SARS-CoV-2 S protein RBD (N501Y), His,Avitag™

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S protein RBDSPD-C52HnSARS-CoV-2  (B.1.1.7/B.1.351/P.1)His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (N501Y), His Tag

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S protein RBDSPD-C52HpSARS-CoV-2  (B.1.351)His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y), His Tag

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S protein RBDSPD-C82E5SARS-CoV-2  (B.1.351)His Tag & Avi TagHEK293Biotinylated SARS-CoV-2 S protein RBD (K417N,E484K,N501Y), His,Avitag™

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S protein RBDSPD-C52HrSARS-CoV-2  (P.1)His TagHEK293SARS-CoV-2 (COVID-19) S protein RBD (K417T, E484K, N501Y), His Tag

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S protein RBDSPD-C82E7SARS-CoV-2  (P.1)His Tag & Avi TagHEK293Biotinylated SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), His,Avitag™

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S1 proteinS1N-C52HgSARS-CoV-2  (B.1.1.7/B.1.351/P.1)His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (N501Y), His Tag

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S1 proteinS1N-C52H9 SARS-CoV-2His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (A570D), His Tag

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S1 proteinS1N-C5256SARS-CoV-2  (B.1.1.7/B.1.351/P.1)His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (D614G), His Tag

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S1 proteinS1N-C82E3SARS-CoV-2  (B.1.1.7/B.1.351/P.1)His Tag & Avi TagHEK293Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His,Avitag™

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S1 proteinS1N-C52HbSARS-CoV-2  (B.1.1.7)His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (P681H), His Tag

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S1 proteinS1N-C52HmSARS-CoV-2  (B.1.351)His TagHEK293SARS-CoV-2 S1 protein (L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D614G), His Tag

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S1 proteinS1N-C52HkSARS-CoV-2  (B.1.1.7)His TagHEK293SARS-CoV-2 (COVID-19) S1 protein (HV69-70del, N501Y, D614G), His Tag

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S2 proteinS2N-C52HcSARS-CoV-2  (B.1.351)His TagHEK293SARS-CoV-2 (COVID-19) S2 protein (A701V), His Tag

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S2 proteinS2N-C52HdSARS-CoV-2  (B.1.1.7)His TagHEK293SARS-CoV-2 (COVID-19) S2 protein (T716I, S982A, D1118H), His Tag

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S2 proteinS2N-C52HeSARS-CoV-2  (P.1)His TagHEK293SARS-CoV-2 (COVID-19) S2 protein (T1027I, V1176F), His Tag

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Nucleocapsid proteinNUN-C52H8SARS-CoV-2  (B.1.1.7)His TagHEK293SARS-CoV-2 Nucleocapsid protein (D3L, S235F), His Tag

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Click to check more variant proteins information.

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