Llama IgG Fc Fusion Proteins - uniquely designed for llama immunization

The single domain antibody was originally discovered in camels back in 1993. This new class of antibody is heavy chain only, containing a single variable domain (VHH) and two constant domains (CH2, CH3). This rare form of antibody has following unique advantages, which makes it an intriguing alternative to the conventional antibodies when it comes to therapeutic use.

First, because of its small size, these nanobodies are known to be able to reach to the cryptic epitopes of the targets normally not accessible by the much larger traditional antibodies. The stability of these antibodies is much better than the traditional antibodies, which makes it possible to be delivered through oral and maybe ocular routes, in addition to intravenous and subcutaneous injection. The nanobodies can also be easily conjugated with cell-killing agents if needed. Last but not least, from manufacturing standpoint, nanobodies are much easier to produce from a variety of hosts.

In October 2017, Belgium-based pharmaceutical company Ablynx reported positive results for its first-in-class nanobody drug caplacizumab from a phase III trial on acquired thrombotic thrombocytopenic purpura (aTTP) patients. This success clearly proves the potential for nanobody drugs.

Performance of proteins conjugated with llama IgG Fc tag

Fig. 1 Human PD-1, Llama IgG2b Fc Tag, low endotoxin (Cat. No. PD1-H5259) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

Fig. 2 Immobilized Human BCMA, Llama IgG2b Fc Tag (Cat. No. BCA-H5259) at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA Mab with a linear range of 0.4-3 ng/mL (Routinely tested).

Product list of proteins conjugated with llama IgG Fc tag （Exclusive）

Molecule	Cat. No.	Product Description	Structure
BCMA	BCA-H5259	Human BCMA, Llama IgG2b Fc Tag, low endotoxin
CD30	TN8-H5250	Human CD30, Llama IgG2b Fc Tag, low endotoxin
CD38	CD8-H5252	Human CD38, Llama IgG2b Fc Tag, low endotoxin
CD47	CD7-H5251	Human CD47, Llama IgG2b Fc Tag, low endotoxin
LAG-3	LA3-H525c	Human LAG-3, Llama IgG2b Fc Tag, low endotoxin
PD-1	PD1-H5259	Human PD-1, Llama IgG2b Fc Tag, low endotoxin
PD-L1	PDL-H5250	Human PD-L1, Llama IgG2b Fc Tag, low endotoxin
Siglec-2	SI2-H525a	Human Siglec-2, Llama IgG2b Fc Tag, low endotoxin
Siglec-3	CD3-H5259	Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin
Glypican 3	GP3-H5257	Human Glypican 3, Llama IgG2b Fc Tag, low endotoxin
Glypican 3	GP3-C5259	Cynomolgus Glypican 3, Llama IgG2b Fc Tag, low endotoxin
Mucin-1	MU1-H5255	Human Mucin-1, Llama IgG2b Fc Tag, low endotoxin
BAFFR	BAR-H5258	Human BAFFR, Llama IgG2b Fc Tag, low endotoxin
NKp46	NC1-H5255	Human NKp46, Llama IgG2b Fc Tag
CD7	CD7-H5258	Human CD7, Llama IgG2b Fc Tag
IL-3 R alpha	ILA-H5255	Human IL-3 R alpha, Llama IgG2b Fc Tag, low endotoxin
IgG2b Fc	IGB-L5204	Llama IgG2b Fc Protein, Tag Free
CD3E & CD3D	CDD-C5259	Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin
CD3E & CD3D	CDD-H5258	Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin

For subsequent biopanning of phage display library, we recommend the corresponding biotinylated protein, which can be coated on Streptavidin-covered surface, for rapid high-throughput screening.

Product list of recommended biotinylated proteins

Molecule	Cat. No.	Product Description	Structure
BCMA	BC7-H82F0	Biotinylated Human BCMA / TNFRSF17 Protein, Fc Tag, Avi Tag (Avitag™)
CD30	CD0-H82E6	Biotinylated Human CD30 / TNFRSF8 Protein, Avi Tag (Avitag™)
CD38	CD8-H82E7	Biotinylated Human CD38 Protein, Avi Tag (Avitag™)
CD47	CD7-H82E9	Biotinylated Human CD47 Protein, Avi Tag (Avitag™)
LAG-3	LA3-H82F3	Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, (Avitag™)
PD-1	PD1-H82E4	Biotinylated Human PD-1(recommended for biopanning)
PD-L1	PD1-H82E5	Biotinylated Human PD-L1 / B7-H1 (recommended for biopanning), Avi Tag (Avitag™)
Siglec-2	SI2-H82F8	Biotinylated Human Siglec-2, Fc Tag, (Avitag™)
Siglec-3	CD3-H82E7	Biotinylated Human Siglec-3 / CD33 Protein, Avi Tag (Avitag™)