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| ID | Components | Size |
| RAS198-C01 | Pre-coated HSV-2 (strain 333) Envelope Glycoprotein D (gD) Microplate | 1 plate |
| RAS198-C02 | HSV-2 (strain 333) (gD) Antibody Positive Control | 100 μL |
| RAS198-C03 | HSV-2 (strain 333) (gD) Antibody Negative Control | 100 μL |
| RAS198-C04 | HRP-Conjugated Antibody | 50 μL |
| RAS198-C05 | 10×Washing Buffer | 50 mL |
| RAS198-C06 | Dilution Buffer | 50 mL |
| RAS198-C07 | Substrate Solution | 12 mL |
| RAS198-C08 | Stop Solution | 7 mL |
The kit is developed for titer measurement of Anti-HSV-2 Envelope Glycoprotein D (gD) antibody IgG in mouse serum.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.
b) Add the HRP-conjugated antibody diluted by Dilution Buffer to the plate.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
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