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> Protein > Glycoprotein H & Glycoprotein L & Glycoprotein O/gH & gL & gO (HCMV) > GLO-V5243 The protein has a calculated MW of 162.2 kDa. The protein migrates as 180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) is more than 85% and the molecular weight of this protein is around 195-235 kDa verified by SEC-MALS.
Immobilized Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) at 1 μg/mL (100 μL/well) can bind Anti-HCMV gH [HCMV16], Human, IgG1 with a linear range of 0.05-2 ng/mL (QC tested).
Human cytomegalovirus (strain AD169) (HHV-5) Glycoprotein H&L&O, His Tag (Cat. No. GLO-V5243) immobilized on CM5 Chip can bind Human PDGF R alpha, His Tag (Cat. No. PDA-H52H7) with an affinity constant of 2.52 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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