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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 100.7 kDa. The protein migrates as 126 kDa±5 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human VCAM-1 Protein, Fc Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human VCAM-1 Protein, Fc Tag, premium grade (Cat. No. VC1-H5253) is more than 85% and the molecular weight of this protein is around 210-250 kDa verified by SEC-MALS.
Immobilized Human VCAM-1 Protein, Fc Tag, premium grade (Cat. No. VC1-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) with a linear range of 2-31 ng/mL (QC tested).
Immobilized Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human VCAM-1 Protein, Fc Tag, premium grade (Cat. No. VC1-H5253) with a linear range of 0.049-0.781 ng/mL (Routinely tested).
Human VCAM-1 Protein, Fc Tag, premium grade (Cat. No. VC1-H5253) captured on Protein A Chip can bind Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) with an affinity constant between 1.00 nM - 50.0 nM as determined in a SPR assay (Biacore 8K) (QC tested).
The SPR based assay shows batch-to-batch consistency between Acro's GMP and PG VCAM-1.
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