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Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.9 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus , followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 45-55 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) is more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin with an affinity constant of 3.42 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) captured on Biotin CAP-Series S Sensor Chip can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) on SA Biosensor, can bind Herceptin with an affinity constant of 2.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Mouse FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-M82W5) on SA Biosensor, can bind Mouse Serum Albumin, His Tag (Cat. No. MSA-M52H8) with an affinity constant of 0.495 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Kielau Kisalu, Neville et al.
Journal: JCI Insight 2020
Application: BLI
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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