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Human FCGRT&B2M Heterodimer Protein, His Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 11.7 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 33 kDa (FCGRT) and 10 kDa (B2M) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human FCGRT&B2M Heterodimer Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) is more than 90% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Captured Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) on CM5 Chip via Anti-His antibody can bind Herceptin® (Trastuzumab) with an affinity constant of 0.516 μM as determined in a SPR assay (Biacore T200) (QC tested).
Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG1 Fc, Tag Free (Cat. No. FCC-H5214) with an affinity constant of 0.957 μM as determined in SPR assay (Biacore 8K) (Routinely tested).
Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG2 Fc, Tag Free (Cat. No. IG2-H5206) with an affinity constant of 33.3 nM as determined in SPR assay (Biacore 8K) (Routinely tested).
Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) with an affinity constant of 0.683 μM as determined in SPR assay (Biacore 8K) (Routinely tested).
Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG4 Fc, Tag Free (SPR verified) (Cat. No. IG4-H5205) with an affinity constant of 0.715 μM as determined in SPR assay (Biacore 8K) (Routinely tested).
Herceptin immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) with an affinity constant of 0.257 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) on SA Biosensor via Biotin his antibody, can bind Herceptin with an affinity constant of 0.14 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) on AR2G Biosensor, can bind Herceptin with an affinity constant of 0.22 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human Serum Albumin, His,Avitag (Cat. No. HSA-H82E3) on SA Biosensor, can bind Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) with an affinity constant of 4.09 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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