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Your Position: Home > Fc Receptor Proteins > FcRn (FCGRT & B2M) > FCN-B82W3

Biotinylated Bovine FcRn / FCGRT&B2M Heterodimer Protein, His,Avitag™ (MALS & SPR verified)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    MABSol® Biotinylated Bovine FcRn Heterodimer Protein, His,Avitag (FCN-B82W3) is expressed from human HEK293 cells. It contains AA Ala 24 - Pro 298 (FCGRT) & Ile 21 - Leu 118 (B2M) (Accession # Q3T119-1(FCGRT) & P01888-1(B2M)).
    Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
  • Molecular Characterization
    Online(Ala 24 - Pro 298 (FCGRT) & Ile 21 - Leu 118 (B2M)) Q3T119-1 (FCGRT) & P01888-1 (B2M)

    Biotinylated Bovine FcRn Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.5 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit B2M contains no tag. The reducing (R) protein migrates as 35-38 kDa (FCGRT) and 12 kDa (B2M) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Bovine FcRn Heterodimer Protein, His,Avitag (Cat. No. FCN-B82W3) SDS-PAGE gel

Biotinylated Bovine FcRn Heterodimer Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Bovine FcRn Heterodimer Protein, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Bovine FcRn Heterodimer Protein, His,Avitag (Cat. No. FCN-B82W3) is more than 90% and the molecular weight of this protein is around 50-60 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Bovine FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCN-B82W3) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin with an affinity constant of 0.485 nM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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