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Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.7 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 15 kDa (B2M) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (Cat. No. FCM-M82W6) is more than 90% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.
Loaded Biotinylated Mouse FcRn Heterodimer Protein, Avitag,His Tag&Twin-Strep Tag (Cat. No. FCM-M82W6) on SA Biosensor, can bind Herceptin with an affinity constant of 3.38 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
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