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Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 38 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) was greater than 90% as determined by SEC-HPLC.
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 0.369 μM as determined in a SPR assay (Biacore T200) (QC tested).
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) captured on Biotin CAP - Series S sensor Chip can bind Human IgG1 Fc, Tag Free (Cat. No. FCC-H5214) with an affinity constant of 0.728 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) captured on Biotin CAP - Series S sensor Chip can bind Human IgG2 Fc, Tag Free (Cat. No. IG2-H5206) with an affinity constant of 0.113 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) captured on Biotin CAP - Series S sensor Chip can bind Human IgG3 Fc, Tag Free (Cat. No. IG3-H5200) with an affinity constant of 3.32 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) captured on Biotin CAP - Series S sensor Chip can bind Human IgG4 Fc, Tag Free (Cat. No. IG4-H5205) with an affinity constant of 1.06 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) on SA Biosensor, can bind Herceptin with an affinity constant of 0.22 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human FCGRT&B2M Heterodimer Protein, Avitag,His Tag&Strep II Tag (Cat. No. FCM-H82W4) on SA Biosensor, can bind Human Serum Albumin, His Tag (Cat. No. HSA-H5220) with an affinity constant of 0.772 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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