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The kit is developed for quantitative detection of natural and recombinant human insulin in serum, plasma and cell culture supernatants.
It is for research use only.
| Analyte | Insulin |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 4.69 μIU/mL |
| Range | 4.69 μIU/mL-300 μIU/mL |
| Assay Time | 1.75 hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 uL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Insulin is less than 4.69 μIU/mL.
Serum – Insulin concentrations were measured in 32 human serum samples, which were collected from healthy human subjects. The measurements of 32 samples are shown in the figure below.
High concentrations of human Insulin serum samples were diluted with 1:2, 1:4, 1:8, 1:16 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of Insulin was 107.8%.
10 replicates of each of 4 samples containing different Insulin concentrations were tested in one assay. Acceptable criteria: CV＜10%.
4 samples containing different concentrations of Insulin were tested in the independent assays. Acceptable criteria: CV<15%.
Insulin was spiked into 3 human serum samples, and then analyzed. The average recovery of Insulin for serum samples is 103.8%.
| ID | Components | Size |
| CEA205-C01 | Pre-coated Anti-Insulin Antibody Microplate | 1 plate |
| CEA205-C02 | Human Insulin Standard (Std.-1) | 300 μIU/mL |
| CEA205-C03 | Biotin-Anti-Insulin Antibody Con. Solution | 400 μL |
| CEA205-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA205-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA205-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA205-C07 | 20× Washing Buffer | 50 mL |
| CEA205-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA205-C09 | Substrate Solution | 12 mL |
| CEA205-C10 | Stop Solution | 6 mL |
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