Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Protein > TEV protease > TEE-V5143

TEV Protease (active enzyme, HPLC verified)

Order Now

  • Product Details
    TEV Protease is a recombinant form of Tobacco Etch Virus protease expressed in Escherichia coli. This protease is used to cleave affinity tags from fusion proteins. The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQS/G, the cleavage site is between Q and S/G. TEV protease contains N-terminus His-tag, it can be removed by Ni2+ affinity resin for purification of the target protein.
  • Application

    Removal of affinity purification tags from fusion proteins.

  • Unit Definition
    1 unit of TEV Protease will cleave 2 µg of MBP-fusion protein to 95% completion in a total reaction volume of 10 µl in 1 hour at 30°C in 50 mM Tris-HCl (pH 7.5 @ 25°C) with 0.5 mM EDTA and 1 mM DTT.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Enzyme Activity
    >1 U/μL
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 250 mM NaCl, 0.1% TritonX-100, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with blue ice, please inquire the shipping cost.

  • Storage

    This product is stable after storage at:

    1. The product MUST be stored at -20°C or lower upon receipt;
    2. -20°C for 3 months under sterile conditions.
SDS-PAGE
TEV protease SDS-PAGE

The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-HPLC
TEV protease SEC-HPLC

The purity of TEV Protease (Cat. No. TEE-V5143) was greater than 90% as determined by SEC-HPLC.

Bioactivity

Two fold dilutions of TEV Protease are incubated with 2 μg MBP5-TEV-paramyosin ΔSal and 1X TEV Protease Reaction Buffer in a 10 µl reaction. The reaction mix is incubated at 30°C for 1 hour. Separation of reaction products are visualized by SDS-PAGE.

  • Clinical and Translational Updates

Comments (0)


ETA of in-stock products: 2 business days

Price(USD) : $105.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message