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Use the kit after you extract host cell DNA from test samples. To achieve the best performances, resDNA Sample Preparation Kit Ⅱ(Magnetic Beads) (Cat. No. OPA-R024) is recommended for DNA extraction.
The kit is used for quantitative detection of residual Pichia pastoris DNA in biopharmaceuticals.
For use in quality control/manufacturing process only.
It is for research use only.
Figure 1. High sensitivity and broad dynamic range using the Pichia pastoris resDNA Quantitation Kit. (A) Typical analysis results obtained with Standard 1 (300 pg/μL) to 6 (3 fg/μL). (B) The standard curve of the 10-fold dilution series. PCR efficiency should be 90-110%.
Figure 2. Assay specificity. Standard curves generated using 10-fold serial dilution of Pichia pastoris genomic DNA (included in the kit) in the presence of DNA (CHO, E. coli and HEK293).
| ID | Components | Size |
| OPA-R017-01 | 2×qPCR Master Mix | 1.0 mL×2 |
| OPA-R017-02 | Pichia Primer & Probe Mix | 700 μL |
| OPA-R017-03 | Pichia DNA Control（3ng/μL） | 100 μL |
| OPA-R017-04 | Dilution Buffer | 1.5 mL×3 |
| OPA-R017-05 | DNase/RNase-free Water | 1.0 mL |
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