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| ID | Components | Size |
| RAS146-C01 | Pre-coated Anti-SARS-CoV-2 Spike RBD Antibody Microplate | 1 plate |
| RAS146-C02 | SARS-CoV-2 Spike RBD (XBB.1.5) | 15 μg |
| RAS146-C03 | Biotin-Anti-SARS-CoV-2 Spike RBD Antibody | 100 μL |
| RAS146-C04 | Streptavidin-HRP | 10 μg |
| RAS146-C05 | 10xWashing Buffer | 50 mL |
| RAS146-C06 | Dilution Buffer | 50 mL |
| RAS146-C07 | Substrate Solution | 12 mL |
| RAS146-C08 | Stop Solution | 7 mL |
This kit is developed for quantitative detection of SARS-CoV-2 Spike RBD (XBB.1.5) in samples.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 6 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the SARS-CoV-2 Spike RBD as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add the Secondary antibody biotin-Anti-SARS-CoV-2 Spike RBD Antibody diluted by Dilution Buffer to the plate.
d) Add the diluted Streptavidin-HRP to the plate.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
Detection of SARS-CoV-2 Spike RBD (XBB.1.5) by sandwich-ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody can bind SARS-CoV-2 Spike RBD (XBB.1.5). Detection was performed using Biotin-Anti-SARS-CoV-2 Spike RBD Antibody with sensitivity of 1.56 ng/mL (QC tested).
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