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Your Position: Home > Viral Proteins > Spike RBD > RAS-T052

Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike RBD)

For research use only.
Materials Provided
IDComponentsSize
RAS052-C01Pre-coated SARS-CoV-2 Spike RBD Microplate1 plate
RAS052-C02Positive Control100 μL
RAS052-C03Negative Control100 μL
RAS052-C04HRP-Anti-Human IgM100 μL
RAS052-C0510xWashing Buffer50 mL
RAS052-C06Dilution Buffer50 mL
RAS052-C07Substrate Solution12 mL
RAS052-C08Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for titer measurement of Anti-SARS-CoV-2 Antibody IgM (Spike RBD) in human serum.
    It is for research use only.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 IgM in serum by SARS-CoV-2 Spike RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control,an HRP-Anti-Human IgM secondary antibody and related buffer.

    Your experiment will include 4 simple steps: a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    b) Add a diluted Secondary antibody HRP-Anti-Human IgM to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike RBD)Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay Kit (Spike RBD) (Cat. No. RAS-T052) ELISA bioactivity

For determination of antibody titer: Determination of antibody titer: the positive sample was diluted with a gradient, and the antibody titer of the sample corresponds to the highest dilution factor that still yields a positive reading.

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Price(USD) : $580.00

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