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Your Position: Home > Antibody > FMC63 > FM3-Y45A1

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  • Source

    Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • Clone

    Y45

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Human

  • Immunogen

    FMC63.

  • Specificity

    Specifically recognizes the antigen-recognition domain of FMC63 derived antibody.

  • Application
    ApplicationRecommended Usage
    ELISA6.25-50 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 6 months under sterile conditions after reconstitution.
SDS-PAGE
FMC63 SDS-PAGE

Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
FMC63 MALS images

The purity of Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) is more than 95% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

Bioactivity-ELISA
 FMC63 ELISA

Immobilized FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) with a linear range of 0.1-6 ng/mL. FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) is verified not recoginized by Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) in low concentration (QC tested).

 FMC63 ELISA

ELISA analysis shows that the binding of Human CD19, Fc Tag (Cat. No. CD9-H5251) to FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) was inhibited by increasing concentration of Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1). The concentration of Human CD19, Fc Tag used is 5 μg/mL (100 μL/well). The IC50 is 0.084 μg/mL (Routinely tested).

Bioactivity-SPR
 FMC63 SPR

Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Cat. No. FM3-Y45A1) captured on CM5 chip via anti-mouse antibodies surface can bind FMC63 scFv with an affinity constant of 1.08 nM as determined in a SPR assay (Routinely tested).

  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
  • Clinical and Translational Updates

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