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Biotinylated Monoclonal Anti-IFNγ Antibody, Mouse IgG1 (13E6H6) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.
13E6H6
Mouse
Mouse IgG1/kappa
Biotin
Hybridoma Monoclonal
Human
Recombinant Human IFNγ is expressed from human HEK293 cells.
This product is a specific antibody specifically reacts with IFN-γ, Human. No cross-reactivity is detected with other human cytokines, including IL-2, IL-4, IL-6, IL-10, GM-CSF and TNF-alpha.
Application | Recommended Usage |
ELISA | 0.2-50 mg/mL |
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Monoclonal Anti-IFNγ Antibody, Mouse IgG1 (13E6H6) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Monoclonal Anti-IFNγ Antibody, Mouse IgG1 (13E6H6) (Cat. No. IFN-BS138) is more than 90% and the molecular weight of this protein is around 140-170 kDa verified by SEC-MALS.
Immobilized Human IFN-gamma, premium grade (Cat. No. IFG-H4211) can bind Biotinylated Monoclonal Anti-IFNγ Antibody, Mouse IgG1 (13E6H6) (Cat. No. IFN-BS138) with a linear range of 0.05-3.125 ng/mL (QC tested).
Biotinylated Monoclonal Anti-IFNγ Antibody, Mouse IgG1 (13E6H6) (Cat. No. IFN-BS138) captured on CM5 chip via anti-mouse antibodies surface can bind Human IFN-gamma, premium grade (Cat. No. IFG-H4211) with an affinity constant of 1.28 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Biotinylated Monoclonal Anti-IFNγ Antibody, Mouse IgG1 (13E6H6) (Cat. No. IFN-BS138) on AMC Biosensor, can bind Human IFN-gamma, premium grade (Cat. No. IFG-H4211) with an affinity constant of 0.268 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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