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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 40.6 kDa. The protein migrates as 45-60 kDa under reducing (R) condition, and 90-130 kDa,150kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD28H, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human CD28H, Fc Tag (Cat. No. CDH-H5251) is more than 90% and the molecular weight of this protein is around 86-106 kDa verified by SEC-MALS.
Immobilized Human B7-H7, His Tag (Cat. No. B77-H52H6) at 5 μg/mL (100 μL/well) can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with a linear range of 0.6-10 ng/mL (QC tested).
Loaded Human B7-H7, His Tag on HIS1K Biosensor, can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with an affinity constant of 16.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Cynomolgus B7-H7, His Tag (Cat. No. B77-C52H3) on HIS1K Biosensor, can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with an affinity constant of 13.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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