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Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4D2) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
4D2
Human IgG1 | Human Kappa
Unconjugated
Recombinant Monoclonal
Virus
Recombinant Rotavirus A (strain RVA/Human/United States/Wa/1974/G1P1A[8]) VP4 Protein is expressed from human 293 cells.
Specifically recognizes Rotavirus A VP4.
| Application | Recommended Usage |
| ELISA | 0.1-63 ng/mL |
>95% as determined by SDS-PAGE.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4D2) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Rotavirus A VP4 Protein, His Tag (Cat. No. VP4-R5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4D2) (Cat. No. VP4-M762) with a linear range of 0.1-2 ng/mL (QC tested).
Monoclonal Anti-Rotavirus A VP4 Antibody, Human IgG1 (4D2) (Cat. No. VP4-M762) captured on Protein A Chip can bind Rotavirus A VP4 Protein, His Tag (Cat. No. VP4-R5243) with an affinity constant of 1.07 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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