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Monoclonal Anti-HA-H1N1(Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
1C10
Human IgG1 | Human Kappa
Unconjugated
Recombinant Monoclonal
Virus
This product is a specific antibody specifically reacts with HA.
| Application | Recommended Usage |
| Western Blot | 1:100-1:1000 |
| ELISA | 1.6-12.5 ng/mL |
>95% as determined by SDS-PAGE.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
This product can cross in Elisa with
Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
Influenza A (A/Georgia/12/2022) Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H7).
This product No cross-reactivity in ELISA with Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6).
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No.HA6-V52H3).
Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H7).
Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3).
Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3).
Influenza B (B/Singapore/INFTT-16-0610/2016) Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H5).
Influenza B (B/Singapore/WUH4618/2021) Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H6).
Influenza A (A/Sydney/1304/2022) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H9).
Monoclonal Anti-HA-H1N1(Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H1N1(Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) (Cat. No. HA1-M757) with a linear range of 0.05-2 ng/mL (QC tested).
Monoclonal Anti-HA-H1N1 (Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) (Cat. No. HA1-M757) captured on Protein A Chip can bind Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) with an affinity constant of 0.334 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Detection of Anti-HA-H1N1(Influenza ASydney52021)-1C10,Human,Human IgG1 Human Kappa by Western Blot. Anti-HA-H1N1(Influenza ASydney52021)-1C10,Human,Human IgG1 Human Kappa at 1/1000 dilution + Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (MALS verified) at 200ng & Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (MALS verified) at 200ng & Influenza A [Sydney/5/2021 (H1N1)] Hemagglutinin (HA) Protein, His Tag (MALS verified) at 200ng.
Secondary: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 95 kDa&100 kDa&100 kDa 12% Bis-Tris Protein Gel.
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