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Your Position: Home > Kits > AAV8 > PAV-A008

Anti-AAV8 Antibody ELISA Kit

For research use only.

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Product Details
Assay TypeSandwich-ELISA
AnalyteAAV8
Format96T
Reactivity/
Regulatory StatusRUO
Sensitivity≤100ng/mL
Standard Curve Range0.938 ng/mL-60 ng/mL
Assay Time3 hr 20 min
Suitable Sample TypeFor the quantitative determination of anti-AAV8 antibodies in serum.
Sample volume100 uL
Materials Provided
IDComponentsSize
PAV008-C01Pre-coated AAV8 Capsid Protein Microplate1 plate
PAV008-C02Anti-AAV8 Antibody Standard2.5 μg
PAV008-C03Biotin-AAV8 Capsid Protein1 μg
PAV008-C04Streptavidin-HRP10 μg
PAV008-C0510xWashing Buffer 50 mL
PAV008-C062xDilution Buffer50 mL
PAV008-C07Substrate Solution12 mL
PAV008-C08Stop Solution7 mL
  • Background
    Adeno-associated virus (AAV) has become one of the most important gene vectors in the field of gene therapy due to its long-term expression, low toxicity, low immunogenicity, and high tissue specificity.Most successful AAV gene therapies for preclinical and clinical studies are limited to natural serotypes, but the presence of neutralizing antibodies against AAV remains a significant barrier to systemic delivery.
  • Application

    Anti-AAV8 Antibody ELISA Kit is developed for the detection of anti-AAV8 antibodies in serum. It can be used for immunogenicity studies and enrollment screening.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a sandwich-ELISA format, providing a rapid detection of Anti-AAV8 Antibody. The kit consists of Pre-coated AAV8 Capsid Protein Microplate, Anti-AAV8 Antibody Standard, Biotin-AAV8 Capsid Protein, Streptavidin-HRP and buffers.

    Your experiment will include 6 simple steps:

    a) All reagents were returned to room temperature(20℃-25℃) before use.

    b) Add your sample to the plate, take the Anti-AAV8 Antibody as standard sample. The samples and standard sample are diluted by Dilution Buffer.

    c) Wash the plate and add the Biotin-AAV8 Capsid Protein diluted by Dilution Buffer to the plate.

    d) Wash the plate and add the diluted Streptavidin-HRP diluted by Dilution Buffer to the plate.

    e) Wash the plate and add TMB.

    f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of Antibody bound.

Typical Data Please refer to DS document for the assay protocol.
 AAV8 TYPICAL DATA

Detection of anti-AAV8 antibodies by sandwich-ELISA Assay.
Immobilized AAV8 Capsid Protein can bind Anti-AAV8 Antibody in 1:50 human serum, and then add Biotin-AAV8 Capsid Protein. Detection was performed using Streptavidin-HRP with sensitivity of 46.9 ng/mL.

 AAV8 TYPICAL DATA

The specific binding of PAV-A008 to Anti-AAV3/5/9 antibody was detected. The results showed that PAV-A008 only binds specifically to Anti-AAV8 antibody.

Validation
Intra-Assay Statistics

Three samples of known concentration in blank human serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.

 AAV8 INTRA-ASSAY STATISTICS

Three samples of known concentration in blank mouse serum were tested six times on one plate to assess intra-assay precision, Intra-Assay Precision CV≤15%.

 AAV8 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Three samples of known concentration in blank human serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.

 AAV8 INTER-ASSAY STATISTICS

Three samples of known concentration in blank mouse serum were tested in six separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.

 AAV8 INTER-ASSAY STATISTICS
  • Clinical and Translational Updates

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