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Your Position: Home > Kits > T7 RNA polymerase > RES-A018

resDetect™ T7 RNA Polymerase ELISA Kit (Residue Testing)

For research use only.

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Product Details
Assay TypeSandwich-ELISA
AnalyteT7 RNA polymerase
Format96T
ReactivityHuman
Regulatory StatusRUO
Sensitivity<0.78 ng/Ml
Standard Curve Range0.78 ng/mL-50 ng/mL
Assay Time3 hr 20 min
Suitable Sample TypeFor the quantitative determination of T7 RNA polymerase in Cell Culture Supernatants.
Sample volume100 uL
Materials Provided
IDComponentsSize
RES018-C01Pre-coated Anti-T7 RNA polymerase Antibody Microplate1 plate
RES018-C02T7 RNA polymerase Standard100 μL
RES018-C03Biotin-Anti-T7 RNA polymerase Antibody150 μL
RES018-C04Streptavidin-HRP50 μL
RES018-C0520xWashing Buffer 50 mL
RES018-C06Biotin-Antibody and Streptavidin-HRP Dilution Buffer50 mL
RES018-C07Standard and Sample Dilution Buffer (5x)30 mL
RES018-C08Substrate Solution12 mL
RES018-C09Stop Solution7 mL
  • Background
    T7 RNA Polymerase ELISA Kit (Residue Testing) is based on ELISA sandwich method and designed to detect and quantitative determine of T7 RNA Polymerase in mRNA preparation processing. It contains T7 RNA Polymerase and a pair of antibodies against the recombinant enzyme, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of T7 RNA Polymerase (ACROBiosystems, cat#T7E-E5143) concentrations. The specificity has been verified.
  • Application

    T7 RNA Polymerase ELISA Kit (Residue Testing)was developed for the detection and quantitative determination of T7 RNA Polymerase in mRNA preparation processing where T7 RNA Polymerase is used as an row agent.

    It is for research use only.

  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of T7 RNA polymerase. The kit consists of Pre-coated Anti-T7 RNA polymerase Antibody Microplate and T7 RNA polymerase Standard and Biotin-Anti-T7 RNA polymerase Antibody and Streptavidin-HRP and buffers.

    Your experiment will include 6 simple steps:

    a) Bring all reagents to room temperature(20℃-25℃) before use.

    b) Add your sample to the plate and take the T7 RNA polymerase as standard. The samples and standard are diluted by Dilution Buffer.

    c) Add the Biotin-Anti-T7 RNA polymerase Antibody diluted by Dilution Buffer to the plate.

    d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

    e) Wash the plate and add TMB.

    f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound enzyme.

Typical Data Please refer to DS document for the assay protocol.
 T7 RNA polymerase TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

Validation
Intra-Assay Statistics

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV<10%.

 T7 RNA polymerase INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.

 T7 RNA polymerase INTER-ASSAY STATISTICS
Recovery

Three T7 RNA polymerases with different concentrations were tested to calculate the recovery rate.

 T7 RNA polymerase RECOVERY
  • Clinical and Translational Updates

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