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Your Position: Home > Kits > Spike protein > RAS-T027

Anti-SARS-CoV-2 (B.1.1.7) Antibody IgG Titer Serologic Assay Kit (Spike Trimer)

For research use only.

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IDComponentsSize
RAS027-C01Pre-coated SARS-CoV-2 Spike Trimer(B.1.1.7) Microplate1 plate
RAS027-C02Positive Control100 μL
RAS027-C03Negative Control100 μL
RAS027-C04HRP-Anti-Human IgG200 μL
RAS027-C0510xWashing Buffer50 mL
RAS027-C06Dilution Buffer50 mL
RAS027-C07Substrate Solution12 mL
RAS027-C08Stop Solution7 mL
  • Background
    Multiple variants of SARS-CoV-2 are circulating globally and posting new challenges to human health. The variant B.1.1.7 identified in the United Kingdom (UK) carries a large number of mutations and is reported to spread more easily and quickly than other variants. There are 9 mutations on the spike protein of the U.K. variant: HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H. To evaluate the impacts of the new variants, a rapid and effective assay kit detecting the levels of antibody against the mutant is in urgent need.
  • Application

    This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 Spike antibody in serum/plasma in vitro. The Spike antigen used in this kit contains 9 mutations (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) found in the U.K. variant (known as B.1.1.7, 20B/501Y.V1 or VOC 202012/01).

    It is for research use only.

  • Storage & Shipping
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Shipping Statement

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike Protein. The kit consists of Pre-coated SARS-CoV-2 Spike Protein Microplate, an Positive Control, an Negative Control, an HRP-Anti-Human IgG secondary antibody and related buffer.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data Please refer to Ds document for the assay protocol.
 Spike protein TYPICAL DATA

Detection of Monoclonal Anti-SARS-CoV-2 Antibody IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Trimer at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG in 1:800 human serum. Detection was performed using HRP-Anti-human IgG antibody with sensitivity of 78 ng/mL (QC tested).

  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $650.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Approved

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